人肾上皮细胞,Human Primary Kidney Epithelial Cells_价格-厂家-供应...

来自 : 发布时间：2024-11-24

您知悉并同意，丁香通平台展示产品并非为平台自身产品，您发起询价或试用申请后，平台将会把您已提供或按要求提供的相关信息同步提供至所咨询的具体产品商家，由商家为您提供具体产品的价格咨询或产品试用注意事项及相关试用产品或完成相应购买，因此您知悉并授权平台将您的信息进行同步共享。您有权拒绝，但您知悉将无法参加询价或试用活动。 如果您查看产品详情，系统显示\"该产品已下架”，说明商品已被卖家或平台下架，暂时无法购买。如您需要购买，建议您可直接发布求购，万千商家快速帮您找货。 如您购买的商品已下架，需要进行售后处理，建议您联系卖家沟通协商或前往丁香通用户服务中心寻求其他帮助。 Cat.#H-6034人肾上皮细胞，Human Primary Kidney Epithelial Cells。每管细胞数量至少1x106cells/ml。 推荐培养基：M6621运输条件： 干冰运输、 液氮保存产品说明链接：http://cellbiologics.com/document/1419013863.pdf​                                                                Product DescriptionHuman Primary Kidney Epithelial Cells from Cell Biologics are isolated from normal human kidney tissue. Human Primary Kidney Epithelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Cell Biologics’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 2 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x106 cells per ml. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. Cells are incubated with EpCAM-1 (CD326) antibody, following the application of magnetic beads pre-coated with secondary antibody.Product TestingHuman Primary Kidney Epithelial Cells from Cell Biologics are tested for expression of markers using antibody, E-cadherin (Catalog No. 610182 BD) or ZO-1 Rabbit Polyclonal Antibody (Catalog No. 617300; Life Technologies) by immunofluorescence staining. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. Per request, a Certificate of Analysis will be provided for each cell lot purchased. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.Laboratory ApplicationsHuman Primary Kidney Epithelial Cells can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.Storage of Cell Biologics’ ProductsCell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request.Never can primary cells be kept at -20 °C.Authorized Uses of Cell Biologics’ ProductsHuman Primary Kidney Epithelial Cells from Cell Biologics are distributed for laboratory research purposes only. Our products are not authorized for human use, not for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any Cell Biologics’cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.DisclaimerAppropriate safety procedures should always be used with this material. Although Human Primary Kidney Epithelial Cells are isolated from normal human tissue, investigators should handle the cells that they receive from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online athttp://www.cdc.gov/biosafety/publications/bmbl5/index.htm.Warranty and LiabilityCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ cells withCell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.  风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。